The difference of osteoblast number between day 5 and day 10 after orthodontic force in tension area and compression area

Abstract

Background: Orthodontic tooth movement can occur through the bone remodeling process. Bone remodeling is a process in which the bone undergoes resorption in the pressure area and apposition in the tension area. Cells that play a role in the process of apposition or bone formation are osteoblasts. Objective: This study aims to determine the difference amount of osteoblast on day 5 and day 10, the difference amount of osteoblast on the tension and pressure areas, and the interaction between the periodontal tissue area and the day after the orthodontic force application. Method: The subjects consisted of six Cavia cobaya which applied orthodontic wire with coil spring and metal ring to separate the two maxillary incisors. The given force is 17.5 grams on each tooth. Subjects were divided
into groups: groups with treatment for 5 days and groups with treatment for 10 days. Euthanasia was done to three guinea pigs from each group on the 5th dan 10th day post orthodontic force appliance. The tissue was then taken to prepare a histological specimen and stained with Haematoxylin Eosin. Osteoblast counts were observed under a light microscope with 400X magnification and Optilab Viewer. One-Way ANOVA and Post Hoc Tukey analyzed observational data. Results: One-Way ANOVA test results showed a significant difference (p < 0.05) in the mean number of osteoblasts between groups. Post Hoc Tukey test results showed a significant difference (p < 0.05) in the mean number of osteoblasts between groups. Conclusion: There was a difference amount of osteoblast on day 5 and day 10, there were differences amount of osteoblast on the tension and pressure areas, and there was an interaction between the periodontal tissue area and the day after the application of orthodontic force.